Transgenic Cre recombinase expression in germ cells and early embryogenesis directs homogeneous and ubiquitous deletion of loxP-flanked gene segments.

Abstract excerpt

We report on the establishment of a transgenic mouse line expressing Cre recombinase under control of the c-kit promoter. Expression of Cre recombinase was only observed in late spermatogenesis and oogenesis, however, Cre-mediated deletion of floxed gene segments occurred at this stage as well as...

Topics

• Alleles
• Animals
• Bacteriophage P1
• Embryo, Mammalian
• Female
• Gene Expression Regulation, Developmental
• Germ Cells
• Integrases
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Transgenic
• Mosaicism
• Mutagenesis, Site-Directed
• Oogenesis
• Polymorphism, Restriction Fragment Length
• Promoter Regions, Genetic
• Proto-Oncogene Proteins c-kit