In vivo mutagenesis of the Hoxb8 hexapeptide domain leads to dominant homeotic transformations that mimic the loss-of-function mutations in genes of the Hoxb cluster.

Abstract excerpt

Hox proteins are transcription factors that control developmental pathways along the anteroposterior axis of vertebrates. On their own, Hox proteins bind DNA weakly, but they gain specificity and affinity by interaction with members of the PBC subfamily of homeobox proteins. In vitro studies indicate that most of these interactions are mediated by the conserved hexapeptide motif of the Hox proteins. To study the...

Topics

• Amino Acid Sequence
• Animals
• Body Patterning
• Bone and Bones
• Embryo, Mammalian
• Female
• Gene Targeting
• Homeodomain Proteins
• Humans
• In Situ Hybridization
• Male
• Mice
• Mice, Transgenic
• Molecular Sequence Data