Article
Asparaginyl-tRNA synthetase from the Escherichia coli temperature-sensitive strain HO202. A proline replacement in motif 2 is responsible for a large increase in Km for asparagine and ATP.
FEBS letters - 24 Mar 1992
Madern D, Anselme J, Härtlein M
Abstract excerpt
The Escherichia coli K12 mutant gene, asnS40, coding for asparaginyl-tRNA synthetase (AsnRS) in the temperature-sensitive strain HO202, was isolated from genomic DNA using the Polymerase Chain Reaction. DNA sequencing revealed that the mutant enzyme differs from the wild-type AsnRS by two amino acids, but only the P231L replacement leads to a change in aminoacylation activity. In the ATP-PPi exchange reaction at...
Topics
- Acylation
- Adenosine Triphosphate
- Amino Acid Sequence
- Amino Acyl-tRNA Synthetases
- Asparagine
- Aspartate-tRNA Ligase
- Escherichia coli
- Kinetics
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction