Article
A high-throughput assay for screening L- or D-amino acid specific aminotransferase mutant libraries.
Analytical biochemistry - 15 Oct 2013
Walton Curtis J W, Chica Roberto A
Abstract excerpt
Aminotransferases are pyridoxal phosphate-dependent enzymes whose potential for the biocatalytic production of enantiopure amino acids is increasingly recognized. Because of this, there is a growing interest in engineering them to alter their substrate specificity and to increase their catalytic activity. Here, we report the development of a high-throughput assay for screening α-ketoglutarate-dependent...
Topics
- Bacillus
- Enzyme Assays
- Escherichia coli
- High-Throughput Screening Assays
- Ketoglutaric Acids
- Mutagenesis
- Mutation
- Protein Engineering
- Substrate Specificity
- Transaminases