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Article

Probing the function of a ligand-modulated dynamic tunnel in bifunctional proline utilization A (PutA).

Archives of biochemistry and biophysics - 15 Nov 2021

Korasick David A, Christgen Shelbi L, Qureshi Insaf A, Becker Donald F, Tanner John J

Abstract excerpt

In many bacteria, the reactions of proline catabolism are catalyzed by the bifunctional enzyme known as proline utilization A (PutA). PutA catalyzes the two-step oxidation of l-proline to l-glutamate using distinct proline dehydrogenase (PRODH) and l-glutamate-γ-semialdehyde dehydrogenase (GSALDH) active sites, which are separated by over 40 Å and connected by a complex tunnel system. The tunnel system consists...

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Identifiers

PMID: 34506758
DOI: 10.1016/j.abb.2021.109025
PMCID: PMC8559791

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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