Article
Reengineering substrate specificity of E. coli glutamate dehydrogenase using a position-based prediction method.
Biotechnology letters - 1 Apr 2017
Geng Feng, Ma Cheng-Wei, Zeng An-Ping
Abstract excerpt
OBJECTIVE: To re-engineer the active site of proteins for non-natural substrates using a position-based prediction method (PBPM). RESULTS: The approach has been applied to re-engineer the E. coli glutamate dehydrogenase to alter its substrate from glutamate to homoserine for a de novo 1,3-propanediol biosynthetic pathway. After identification of key residues that determine the substrate specificity, residue K92...
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