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Article

Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.

The Journal of cell biology - 5 Jun 2017

Malinová Anna, Cvačková Zuzana, Matějů Daniel, Hořejší Zuzana, Abéza Claire, Vandermoere Franck, Bertrand Edouard, Staněk David, Verheggen Céline

Abstract excerpt

Splicing is catalyzed by the spliceosome, a complex of five major small nuclear ribonucleoprotein particles (snRNPs). The pre-mRNA splicing factor PRPF8 is a crucial component of the U5 snRNP, and together with EFTUD2 and SNRNP200, it forms a central module of the spliceosome. Using quantitative proteomics, we identified assembly intermediates containing PRPF8, EFTUD2, and SNRNP200 in association with the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 28515276
DOI: 10.1083/jcb.201701165
PMCID: PMC5461031

Genes

PRPF8
EFTUD2
ZNHIT2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

Related research

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Research trail→

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Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.PMID 28515276Read ↗

Retinitis pigmentosa-linked mutations impair the snRNA unwinding activity of SNRNP200 and reduce pre-mRNA binding of PRPF8.Semantic proximity Read ↗

prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast.Semantic proximity Read ↗

Activation of Prp28 ATPase by phosphorylated Npl3 at a critical step of spliceosome remodeling.Semantic proximity Read ↗

Structural and functional analysis of essential pre-mRNA splicing factor Prp19p.Semantic proximity Read ↗

The human U5 snRNP 52K protein (CD2BP2) interacts with U5-102K (hPrp6), a U4/U6.U5 tri-snRNP bridging protein, but dissociates upon tri-snRNP formationSemantic proximity Read ↗

Identification of a 35S U4/U6.U5 tri-small nuclear ribonucleoprotein (tri-snRNP) complex intermediate in spliceosome assembly.Semantic proximity Read ↗

Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site.Semantic proximity Read ↗

Regulation of constitutive and alternative mRNA splicing across the human transcriptome by PRPF8 is determined by 5' splice site strength.Semantic proximity Read ↗

A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity.Semantic proximity Read ↗

PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa.Semantic proximity Read ↗

Chaperoning the chaperones: Proteomic analysis of the SMN complex reveals conserved and etiologic connections to the proteostasis networkSemantic proximity Read ↗

Structural basis for dual roles of Aar2p in U5 snRNP assembly.Semantic proximity Read ↗

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