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Article

Directed evolution of Thermotoga neapolitana xylose isomerase: high activity on glucose at low temperature and low pH.

Protein engineering - 1 Sept 2003

Sriprapundh Dinlaka, Vieille Claire, Zeikus J Gregory

Abstract excerpt

The Thermotoga neapolitana xylose isomerase (TNXI) is extremely thermostable and optimally active at 95 degrees C. Its derivative, TNXI Val185Thr (V185T), is the most active type II xylose isomerase reported, with a catalytic efficiency of 25.1 s(-1) mM(-1) toward glucose at 80 degrees C (pH 7.0). To further optimize TNXI's potential industrial utility, two rounds of random mutagenesis and low temperature/low pH...

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Identifiers

PMID: 14560054
DOI: 10.1093/protein/gzg082

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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