Article
Selection in vitro of trans-acting genomic human hepatitis delta virus (HDV) ribozymes.
European journal of biochemistry - 1 May 1996
Nishikawa F, Kawakami J, Chiba A, Shirai M, Kumar P K, Nishikawa S
Abstract excerpt
In an effort to identify the functional structure as well as new active variants of the trans-acting genomic ribozyme of human hepatitis delta virus (HDV), we applied an in vitro selection procedure. A total of 14 rounds of selection and amplification was repeated and various mutant ribozymes in G10 and G14 pools analyzed. Active ribozymes which were isolated in the present study (from G10 and G14) all possessed...
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