Article
Detection of K-ras mutations by denaturing gradient gel electrophoresis (DGGE): a study on pancreatic cancer.
Anticancer research - 1 Jan 2000
Pellegata N S, Losekoot M, Fodde R, Pugliese V, Saccomanno S, Renault B, Bernini L F, Ranzani G N
Abstract excerpt
In pancreatic neoplasias mutations in the first exon (codon 12) of K-ras gene occur at high frequency and seem to have a diagnostic significance. We set up the DGGE conditions to search for these mutations in pancreatic tumor sample DNAs. All samples were directly classified by simply comparing t...
Topics
- Adenocarcinoma
- Base Sequence
- Cell Line
- Codon
- Cystadenoma
- DNA, Neoplasm
- Genes, ras
- Humans
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Pancreatic Diseases
- Pancreatic Neoplasms
- Polymerase Chain Reaction
- Tumor Cells, Cultured