Article
Detection of K-ras mutations by denaturing gradient gel electrophoresis (DGGE): a study on pancreatic cancer.
Anticancer research - 1 Jan 2000
Pellegata N S, Losekoot M, Fodde R, Pugliese V, Saccomanno S, Renault B, Bernini L F, Ranzani G N
Abstract excerpt
In pancreatic neoplasias mutations in the first exon (codon 12) of K-ras gene occur at high frequency and seem to have a diagnostic significance. We set up the DGGE conditions to search for these mutations in pancreatic tumor sample DNAs. All samples were directly classified by simply comparing their DGGE patterns with those of control cell lines carrying known K-ras base substitutions. We found a mutation...
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