Article
ME-PCR for the identification of mutated K-ras in serum and bile of pancreatic cancer patients: an unsatisfactory technique for clinical applications.
Clinica chimica acta; international journal of clinical chemistry - 1 Dec 2000
Zambon C, Navaglia F, Basso D, Gallo N, Greco E, Piva M G, Fogar P, Pasquali C, Pedrazzoli S, Plebani M
Abstract excerpt
Our aim was to assess the clinical reliability of mutated K-ras detection in serum or bile for the diagnosis of pancreatic cancer using ME-PCR. DNA was extracted from 1 ml serum obtained from 29 patients with pancreatic cancer and 12 control subjects. ME-PCR was optimized using a mixture of normal DNA added with different amounts of mutated DNA. The analysis of sera obtained from the 29 patients and of bile...
Topics
- Adult
- Aged
- Aged, 80 and over
- Bile
- Codon
- DNA
- DNA Mutational Analysis
- Electrophoresis, Agar Gel
- Female
- Genes, ras
- Humans
- Male
- Middle Aged
- Mutation