Article
What limits affinity maturation of antibodies in Xenopus--the rate of somatic mutation or the ability to select mutants?
The EMBO journal - 1 Dec 1992
Wilson M, Hsu E, Marcuz A, Courtet M, Du Pasquier L, Steinberg C
Abstract excerpt
Although the Xenopus immunoglobulin heavy chain locus is structurally and functionally similar to mammalian IgH loci, Xenopus antibodies are limited in heterogeneity, and they mature only slightly in affinity during immune responses. During the antibody response of isogenic frogs to DNP-KLH, mu and upsilon cDNA sequences using elements of the VH1 family were cloned, sequenced and compared with germline...
Topics
- Amino Acid Sequence
- Animals
- Antibody Formation
- Base Sequence
- DNA
- DNA Mutational Analysis
- Hemocyanins
- Immunoglobulin Heavy Chains
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction