Article
Temperature-sensitive mutants of hsp82 of the budding yeast Saccharomyces cerevisiae.
Molecular & general genetics : MGG - 1 Mar 1994
Kimura Y, Matsumoto S, Yahara I
Abstract excerpt
The budding yeast Saccharomyces cerevisiae has two HSP90-related genes per haploid genome, HSP82 and HSC82. Random mutations were induced in vitro in the HSP82 gene by treatment of the plasmid with hydroxylamine. Four temperature-sensitive (ts) mutants and one simultaneously ts and cold-sensitive (cs) mutant were then selected in a yeast strain in which HSC82 had previously been disrupted. The mutants were found...
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