Article
DRB1*0301 molecules recognize a structural motif distinct from the one recognized by most DR beta 1 alleles.
Journal of immunology (Baltimore, Md. : 1950) - 15 Oct 1992
Sidney J, Oseroff C, Southwood S, Wall M, Ishioka G, Koning F, Sette A
Abstract excerpt
The DR3-restricted peptide, MT 65 kDa 3-13, was used to develop a DR3-specific binding assay. The binding activity detected was strictly pH-dependent, in that it was optimal in the pH 4 to 5 range, and no activity was detected at neutral pH. By means of affinity chromatography purifications and the use of DR3-transfected fibroblasts, it was shown that no cross-reactivity exists at the level of DR52a molecules,...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- Chromosome Mapping
- HLA-DR Antigens
- HLA-DRB1 Chains
- Histocompatibility Antigens Class II
- Hydrogen-Ion Concentration
- Mice
- Molecular Sequence Data