Article
TATA-binding protein activates transcription when upstream of a GCN4-binding site in a novel yeast promoter.
The Journal of biological chemistry - 15 Oct 1992
Brandl C J, Martens J A, Liaw P C, Furlanetto A M, Wobbe C R
Abstract excerpt
In the gal-his3 hybrid promoter, his3-GG1, GCN4 stimulates transcription at the position normally occupied by a TATA element. This expression requires two elements within gal1-10 sequences, a REB1-binding site and a second element, Z, which resides 20 base pairs upstream of the GCN4-binding site. No obvious TATA element is present in this promoter. To characterize the function of Z, we replaced it with short...
Topics
- Alleles
- Base Sequence
- Binding Sites
- Cloning, Molecular
- DNA Probes
- DNA, Fungal
- DNA-Binding Proteins
- Fungal Proteins
- Genes, Fungal
- Molecular Sequence Data
- Mutagenesis, Site-Directed