Article
Three GCN4 responsive elements act synergistically as upstream and as TATA-like elements in the yeast TRP4 promoter.
The EMBO journal - 1 Sept 1990
Mösch H U, Graf R, Schmidheini T, Braus G
Abstract excerpt
The yeast TRP4 promoter contains three responsive elements (GCREs) for the 'general control' transcriptional activator GCN4, which are arranged in two upstream elements, UAS1 (GCRE1) and UAS2 (GCRE2 and GCRE3). A point mutation analysis of these elements revealed that all three GCREs are required for GCN4-dependent transcription, but none are involved in basal transcription. Basal transcription and GCN4-dependent...
Topics
- Alleles
- Base Sequence
- Blotting, Northern
- DNA-Binding Proteins
- Deoxyribonuclease I
- Fungal Proteins
- Gene Expression Regulation, Fungal
- Genes, Regulator
- Molecular Sequence Data
- Mutation
- Nucleotide Mapping