Effects of amino acid replacements within the tetrabasic cleavage site on the processing of the human insulin receptor precursor expressed in Chinese hamster ovary cells.

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Article

Effects of amino acid replacements within the tetrabasic cleavage site on the processing of the human insulin receptor precursor expressed in Chinese hamster ovary cells.

The Journal of biological chemistry - 5 Oct 1990

Yoshimasa Y, Paul J I, Whittaker J, Steiner D F

Abstract excerpt

We have studied the specificity requirements for processing of the human insulin proreceptor by successively replacing each basic amino acid in the tetrabasic cleavage site with alanine. These mutated receptor cDNAs have then been overexpressed in Chinese hamster ovary cells, using vectors contai...

Topics

Amino Acid Sequence
Animals
Base Sequence
Cell Line
Cricetinae
Cricetulus
Female
Humans
Insulin
Kinetics
Molecular Sequence Data
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Oligonucleotide Probes

Ovary

Phosphorylation

Protein Precursors

Protein Processing, Post-Translational

Receptor, Insulin

Source

Open in PubMed

Identifiers

PMID: 2211623

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2211623-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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