Article
Characterization of an insulin receptor mutant lacking the subunit processing site.
The Journal of biological chemistry - 25 May 1990
Williams J F, McClain D A, Dull T J, Ullrich A, Olefsky J M
Abstract excerpt
An insulin receptor mutant was constructed utilizing site-directed mutagenesis to delete the Arg-Lys-Arg-Arg basic amino acid cleavage site (positions 720-723) from the cDNA encoding the human insulin proreceptor. This mutant was transfected into Chinese hamster ovary cells. Immunoprecipitation o...
Topics
- Affinity Labels
- Amino Acid Sequence
- Animals
- Azides
- Base Sequence
- Cell Line
- Chromosome Deletion
- Clone Cells
- Humans
- Insulin
- Kinetics
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Phosphorylation
- Receptor, Insulin
- Transfection