Article
A single amino acid mutation in CDR3 of the 3-14-9 L chain abolished expression of the IDA 10-defined idiotope and antigen binding.
Journal of immunology (Baltimore, Md. : 1950) - 1 Sept 1992
Hall B L, Zaghouani H, Daian C, Bona C A
Abstract excerpt
The ABPC 48 myeloma protein and the 3-14-9 mAb derive their V region genes from the same VH and V kappa gene families. They also share a cross-reactive idiotope defined by the anti-Id mAb IDA 10. Whereas ABPC 48 is specific for bacterial levan, 3-14-9 showed a significant Ag-binding activity to aminophenyl-beta-N-acetylglucosaminide (AZO). In order to define the molecular basis of idiotope expression and...
Topics
- Animals
- Antigens
- Base Sequence
- Epitopes
- Immunoglobulin Idiotypes
- Immunoglobulin Light Chains
- Immunoglobulin Variable Region
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Mutation