Article
In vivo human somatic mutation: frequency and spectrum with age.
Mutation research - 1 Apr 1992
Grist S A, McCarron M, Kutlaca A, Turner D R, Morley A A
Abstract excerpt
The number and molecular nature of in vivo mutations in relation to age was studied at the autosomal HLA-A locus in human lymphocytes. Mutant lymphocytes were isolated by immunoselection, cloned at limiting dilution and enumerated, and the HLA-A gene and other polymorphic gene loci on chromosome 6 were studied by Southern blotting to determine gene dosage and loss of heterozygosity. Results of 167 assays in 73...
Topics
- Adolescent
- Adult
- Aged
- Aging
- HLA Antigens
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Lymphocytes
- Middle Aged
- Mutation
- Recombination, Genetic