Article
Identification of a protein-binding site in the promoter of the human thymidine kinase gene required for the G1-S-regulated transcription.
The Journal of biological chemistry - 5 Feb 1992
Kim Y K, Lee A S
Abstract excerpt
When quiescent cells are stimulated to enter the cell cycle, the thymidine kinase (TK) gene is transcriptionally activated at the border of G1 and S. In this report we show that the human TK promoter contains multiple protein-binding sites. By site-directed mutagenesis, we identified a protein-binding site on the human TK promoter required for conferring G1-S-regulated transcription to a heterologous promoter and...
Topics
- Animals
- Base Sequence
- Binding Sites
- Cell Cycle
- Cell Line
- Cricetinae
- Cricetulus
- DNA Fingerprinting
- DNA-Binding Proteins
- G1 Phase
- Gene Expression Regulation