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Article

The mutational effect of Ile58 at subsite C in hen egg-white lysozyme on substrate binding, enzymatic activity, and protein stability.

Bioscience, biotechnology, and biochemistry - 1 Jan 2013

Kawamura Shunsuke, Chijiiwa Yuki, Torikata Takao, Araki Tomohiro

Abstract excerpt

Ile58 of hen egg-white lysozyme (HEL) is buried in the interior of the molecule and is considered to participate in sugar residue binding at subsite C through hydrophobic interaction. The contribution of Ile58 to lysozyme function and stability was investigated by replacement of Ile58 with less hydrophobic residues, Val (I58V) and Ala (I58A). Replacement of Ile58 with Ala decreased substrate binding ability to an...

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Identifiers

PMID: 23470762
DOI: 10.1271/bbb.120811

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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