Article
Design and structural analysis of an engineered thermostable chicken lysozyme.
Protein science : a publication of the Protein Society - 1 Oct 1995
Shih P, Kirsch J F
Abstract excerpt
A hyperstable (hs) variant of chicken egg-white lysozyme with enhanced thermal (delta Tm approximately +10.5 degrees C) and chemical (delta Cm for guanidine hydrochloride denaturation = +1.3 M) stabilities relative to wild-type (WT) was constructed by combining several individual stabilizing subs...
Topics
- Amino Acid Sequence
- Animals
- Birds
- Calorimetry, Differential Scanning
- Chickens
- Circular Dichroism
- Coturnix
- Crystallography, X-Ray
- Enzyme Stability
- Female
- Genetic Variation
- Guanidine
- Guanidines
- Hot Temperature
- Humans
- Models, Molecular
- Models, Structural
- Muramidase