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Article

Engineered β-Lactoglobulin Produced in E. coli: Purification, Biophysical and Structural Characterisation.

Molecular biotechnology - 1 Oct 2016

Loch Joanna I, Bonarek Piotr, Tworzydło Magdalena, Polit Agnieszka, Hawro Barbara, Łach Aneta, Ludwin Eryk, Lewiński Krzysztof

Abstract excerpt

Functional recombinant bovine β-lactoglobulin has been produced by expression in E. coli using an engineered protein gene and purified to homogeneity by applying a new protocol. Mutations L1A/I2S introduced into the protein sequence greatly facilitate in vivo cleavage of the N-terminal methionine, allowing correctly folded and soluble protein suitable for biochemical, biophysical and structural studies to be...

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Identifiers

PMID: 27380951
DOI: 10.1007/s12033-016-9960-z
PMCID: PMC5035327

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