Article
Recombinant Production of an Inulinase in a Saccharomyces cerevisiae gal80 Strain.
Journal of microbiology and biotechnology - 1 Nov 2010
Lim Seok-Hwan, Lee Hongweon, Sok Dai-Eun, Choi Eui-Sung
Abstract excerpt
The inulinase gene (INU1) from Kluyveromyces marxianus NCYC2887 strain was overexpressed by using GAL10 promotor in a △gal80 strain of Saccharomyces cerevisiae. The inulinase gene lacking the original signal sequence was fused in-frame to mating factor alpha signal sequence for secretory expression. Use of the △gal80 strain allowed the galactose-free induction of inulinase expression using a glucose-only medium....
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