Article
Molecular characterization of a gene encoding extracellular serine protease isolated from a subtilisin inhibitor-deficient mutant of Streptomyces albogriseolus S-3253.
Applied and environmental microbiology - 1 Jan 1995
Taguchi S, Odaka A, Watanabe Y, Momose H
Abstract excerpt
An extracellular serine protease produced by a mutant, M1, derived from Streptomyces albogriseolus S-3253 that no longer produces a protease inhibitor (Streptomyces subtilisin inhibitor [SSI]) was isolated. A 20-kDa protein was purified by its affinity for SSI and designated SAM-P20. The amino acid sequence of the amino-terminal region of SAM-P20 revealed high homology with the sequences of Streptomyces griseus...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- DNA Primers
- Gene Expression Regulation, Enzymologic
- Molecular Sequence Data
- Mutation
- Restriction Mapping
- Sequence Alignment