Article
Effect of inhibitory activity of mutation at reaction site P4 of the Streptomyces subtilisin inhibitor, SSI.
Protein engineering - 1 May 1990
Kojima S, Kumagai I, Miura K
Abstract excerpt
The protein Streptomyces subtilisin inhibitor, SSI, efficiently inhibits a bacterial serine protease, subtilisin BPN'. We recently demonstrated that functional change in SSI was possible simply by replacing the amino acid residue at the reactive P1 site (methionine 73) of SSI. The present paper r...
Topics
- Bacterial Proteins
- Binding Sites
- Kinetics
- Methionine
- Mutation
- Serine Proteinase Inhibitors
- Streptomyces
- Structure-Activity Relationship
- Subtilisins
- Trypsin