Article
Catalytic properties of cholinesterases: importance of tyrosine 109 in Drosophila protein.
Neuroreport - 1 Jan 1992
Mutero A, Pralavorio M, Simeon V, Fournier D
Abstract excerpt
Tyrosine 109 in the acetylcholinesterase sequence of Drosophila melanogaster corresponds to an aspartate in vertebrate cholinesterases. Mutation of this amino acid to a glycine in the human butyrylcholinesterase gives rise to the 'atypic' phenotype characterized by a reduced activity for charged compounds. We investigated the importance of tyrosine 109 in the Drosophila sequence by in vitro mutagenesis and its...
Topics
- Acetylcholinesterase
- Animals
- Catalysis
- Cholinesterase Inhibitors
- DNA
- Drosophila melanogaster
- Electrophoresis, Polyacrylamide Gel
- Gene Expression Regulation
- Hydrogen-Ion Concentration
- Kinetics
- Mutation