Article
A systematic approach to defining the germline gene counterparts of a mutated autoantibody from a patient with rheumatoid arthritis.
Arthritis and rheumatism - 1 Mar 1992
Soto-Gil R W, Olee T, Klink B K, Kenny T P, Robbins D L, Carson D A, Chen P P
Abstract excerpt
OBJECTIVE: To define the germline counterparts of potentially highly mutated autoantibodies in disease states. METHODS: We developed a systematic approach by first characterizing a rearranged Ig gene and its upstream flank, and then designing suitable primers to amplify specifically the putative germline counterpart. RESULTS: We identified and characterized the germline counterpart of a rheumatoid factor heavy...
Topics
- Amino Acid Sequence
- Arthritis, Rheumatoid
- Autoantibodies
- Base Sequence
- DNA Probes
- Gene Rearrangement, B-Lymphocyte, Heavy Chain
- Genes, Immunoglobulin
- Humans
- Immunoglobulin Heavy Chains
- Molecular Sequence Data