Article
Phenotypic conversion of SV40-immortalized human diploid fibroblasts to senescing cells by introduction of an antisense gene for SV40-T antigen.
Cell structure and function - 1 Dec 1992
Tanaka Y, Tang X, Hou D X, Gao H, Kitabayashi I, Gachelin G, Yokoyama K
Abstract excerpt
Normal human lung fibroblast diploid cells, WI-38, become senescent after a definite number of divisions. VA-13 is a line of immortalized cells established by transformation of WI-38 cells by SV40 virus. To determine whether SV40 large T (SV40-T) antigen is essential for this immortalization of W...
Topics
- Antigens, Polyomavirus Transforming
- Cell Cycle
- Cell Transformation, Viral
- Cellular Senescence
- DNA, Antisense
- Fibroblasts
- Gene Expression Regulation, Viral
- Humans
- Phenotype
- Phosphorylation
- Retinoblastoma Protein
- Simian virus 40