Article
Transposon mutagenesis in Legionella pneumophila. II.--Mutants exhibiting impaired intracellular growth within cultured macrophages and reduced virulence in vivo.
Research in microbiology - 1 Jun 1992
Tully M, Williams A, Fitzgeorge R B
Abstract excerpt
Transposon Tn5 mutants of L. pneumophila were isolated and screened for loss of virulence-associated characteristics. Three mutants were found with normal ability to produce putative pathogenicity determinants and to be endocytosed by guinea pig alveolar macrophages in vitro but with a greatly reduced ability to multiply within them. These mutants showed considerable loss of virulence in an authentic animal model...
Topics
- Animals
- Blotting, Southern
- DNA Transposable Elements
- DNA, Bacterial
- Guinea Pigs
- In Vitro Techniques
- Legionella pneumophila
- Macrophages
- Mutagenesis, Insertional
- Nucleic Acid Hybridization