Article
Identification of Legionella pneumophila genes required for growth within and killing of human macrophages.
Infection and immunity - 1 Dec 1993
Sadosky A B, Wiater L A, Shuman H A
Abstract excerpt
Legionella pneumophila was mutagenized with Tn903dIIlacZ, and a collection of mutants was screened for defects in macrophage killing (Mak-). Of 4,564 independently derived mutants, 55 (1.2%) showed a reduced or complete lack in the ability to kill HL-60-derived human macrophages. Forty-nine of the Mak- mutants could be assigned to one of 16 DNA hybridization groups. Only one group (9 of the 10 members) could be...
Topics
- Bacterial Adhesion
- Cell Death
- Cell Line
- DNA Transposable Elements
- DNA, Bacterial
- Genes, Bacterial
- Genetic Complementation Test
- Humans
- Lac Operon
- Legionella pneumophila
- Legionnaires' Disease