Article
DNA sequence of the skeletal muscle calcium release channel cDNA and verification of the Arg615----Cys615 mutation, associated with porcine malignant hyperthermia, in Norwegian landrace pigs.
Animal genetics - 1 Jan 1992
Harbitz I, Kristensen T, Bosnes M, Kran S, Davies W
Abstract excerpt
Porcine calcium release channel (CRC) cDNA from skeletal muscle has been cloned and sequenced. The deduced amino acid sequence showed 97% identity to the corresponding rabbit and human sequences. Using oligonucleotide primers based on the nucleotide sequence, CRC cDNA fragments from seven pigs representing HALNN, HALNn and HALnn genotypes have been amplified. Sequencing and restriction digestion of the amplified...
Topics
- Animals
- Arginine
- Base Sequence
- Calcium Channels
- Cysteine
- DNA
- Malignant Hyperthermia
- Molecular Sequence Data
- Muscles
- Mutation
- Polymerase Chain Reaction