Article
Tissue distribution of ryanodine receptor isoforms and alleles determined by reverse transcription polymerase chain reaction.
The Journal of biological chemistry - 16 Dec 1994
Ledbetter M W, Preiner J K, Louis C F, Mickelson J R
Abstract excerpt
The tissue distribution of mRNA for ryanodine receptor (ryr) isoforms in various porcine tissues has been determined using the reverse transcription-polymerase chain reaction (RT-PCR). First strand cDNA was synthesized from total tissue RNA with reverse transcriptase and random hexamer primers. PCR primers were selected to amplify an approximately 500-base pair segment from homologous regions near the 5' end of...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- Base Sequence
- Brain
- Calcium Channels
- Cloning, Molecular
- DNA Primers
- Gene Expression
- Molecular Sequence Data
- Muscle Proteins
