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Tissue distribution of ryanodine receptor isoforms and alleles determined by reverse transcription polymerase chain reaction.

The Journal of biological chemistry - 16 Dec 1994

Ledbetter M W, Preiner J K, Louis C F, Mickelson J R

Abstract excerpt

The tissue distribution of mRNA for ryanodine receptor (ryr) isoforms in various porcine tissues has been determined using the reverse transcription-polymerase chain reaction (RT-PCR). First strand cDNA was synthesized from total tissue RNA with reverse transcriptase and random hexamer primers. PCR primers were selected to amplify an approximately 500-base pair segment from homologous regions near the 5' end of...

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PMID: 7989322

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