Article
Separation of immortalization from tumor induction with polyoma large T mutants that fail to bind the retinoblastoma gene product.
Oncogene - 1 Oct 1992
Freund R, Bronson R T, Benjamin T L
Abstract excerpt
The mouse polyomavirus encodes a tumor-suppressor gene inactivator in its large T protein and a proto-oncogene activator in its middle T protein. We have used site-directed mutagenesis to selectively inactivate the former function without affecting the latter. Two mutant viruses were constructed to encode altered large T proteins that fail to bind the retinoblastoma tumor-suppressor gene product pRB, along with...
Topics
- Animals
- Antigens, Polyomavirus Transforming
- Base Sequence
- Cell Transformation, Neoplastic
- Cell Transformation, Viral
- Genes, Retinoblastoma
- Mice
- Mice, Inbred C3H
- Molecular Sequence Data
- Mutation
- Neoplasm Proteins