Article
Functional implications of mutations within polyomavirus large T antigen Rb-binding domain: effects on pRb and p107 binding in vitro and immortalization activity in vivo.
Journal of virology - 1 Jul 1996
Pilon A A, Desjardins P, Hassell J A, Mes-Masson A M
Abstract excerpt
In this study, we have extensively modified the Rb-binding domain of polyomavirus large T antigen. Mutant polyomavirus large T antigens were tested for their ability to bind pRb and p107 in vitro and assayed for their capacity to immortalize primary rat embryo fibroblasts in vivo. Polyomavirus la...
Topics
- Amino Acid Sequence
- Animals
- Antigens, Polyomavirus Transforming
- Binding Sites
- Cell Line
- Cell Transformation, Viral
- Humans
- Molecular Sequence Data
- Mutation
- Nuclear Proteins
- Protein Binding
- Rats
- Rats, Inbred F344
- Retinoblastoma Protein
- Retinoblastoma-Like Protein p107