Article
Dissociation of Rb-binding and anchorage-independent growth from immortalization and tumorigenicity using SV40 mutants producing N-terminally truncated large T antigens.
Virology - 1 Sept 1990
Thompson D L, Kalderon D, Smith A E, Tevethia M J
Abstract excerpt
The large T antigen of SV40 is both necessary and sufficient for conversion of primary mouse cells to cells with fully transformed phenotype. In this investigation, the influence of the N-terminal portion of T antigen on individual transformed cell characteristics was probed by using mutants bearing deletions in the 5'T antigen coding sequence. Specifically, DNA constructs expected to produce T antigens missing...
Topics
- Animals
- Antigens, Viral, Tumor
- Cell Line, Transformed
- Chromosome Deletion
- Cloning, Molecular
- DNA Replication
- Mice
- Mice, Inbred C57BL
- Mice, Nude
- Mutation
- Phosphoproteins