Dissociation of Rb-binding and anchorage-independent growth from immortalization and tumorigenicity using SV40 mutants producing N-terminally truncated large T antigens.

Abstract excerpt

The large T antigen of SV40 is both necessary and sufficient for conversion of primary mouse cells to cells with fully transformed phenotype. In this investigation, the influence of the N-terminal portion of T antigen on individual transformed cell characteristics was probed by using mutants bearing deletions in the 5'T antigen coding sequence. Specifically, DNA constructs expected to produce T antigens missing...

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