Article
Ligand-independent reduction of cAMP receptors in Dictyostelium discoideum cells over-expressing a mutated ras gene.
European journal of biochemistry - 1 Sept 1992
Ludérus M E, Kesbeke F, Knetsch M L, Van Driel R, Reymond C D, Snaar-Jagalska B E
Abstract excerpt
Drug-resistance selection in Dictyostelium discoideum transformants resulted in up to eight-times-higher ras protein levels. Over-production of the wild-type ras protein did not lead to an aberrant phenotype. Increased levels of the mutated [G12T]ras protein, however, were correlated with severe deficiencies in aggregation and development. This aberrant phenotype is associated with reduced cAMP binding, due to a...
Topics
- Animals
- Blotting, Northern
- Blotting, Western
- Cyclic AMP
- Dictyostelium
- Drug Resistance, Microbial
- Gene Expression Regulation, Fungal
- Genes, ras
- Gentamicins
- Mutagenesis
- Phenotype