Article
Expression of the rat muscarinic receptor gene m3 in Dictyostelium discoideum.
Die Pharmazie - 1 Oct 1998
Voith G, Kramm H, Zündorf I, Winkler T, Dingermann T
Abstract excerpt
We functionally expressed the rat muscarinic m3 receptor (rm3) in the cellular slime mold Dictyostelium discoideum under the control of the homologous discoidin I gamma promoter. Cells transfected with the authentic rm3 receptor gene expressed about 100 functional receptor molecules per cell, corresponding to a Bmax for [3H]-NMS of 36 +/- 9 fmol/mg of protein in isolated membranes. Genetic fusion of the...
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