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Article

Modification of the apolipoprotein B gene in HepG2 cells by gene targeting.

The Journal of clinical investigation - 1 Jul 1992

Farese R V, Flynn L M, Young S G

Abstract excerpt

The HepG2 cell line has been used extensively to study the synthesis and secretion of apolipoprotein (apo) B. In this study, we tested whether gene-targeting techniques can be used to inactivate one of the apo B alleles in HepG2 cells by homologous recombination using a transfected gene-targeting vector. Our vector contained exons 1-7 of the apo B gene, in which exon 2 was interrupted by a promoterless neomycin...

Topics

Alleles
Antibodies, Monoclonal
Apolipoproteins B
Base Sequence
Carcinoma, Hepatocellular
Genetic Vectors
Humans
Liver Neoplasms
Molecular Sequence Data
Mutation
Radioimmunoassay

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Recombination, Genetic

Tumor Cells, Cultured

Source

Open in PubMed Open full text

Identifiers

PMID: 1321843
DOI: 10.1172/JCI115845
PMCID: PMC443089

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1321843-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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