Analysis of novel apolipoprotein B mutations using a modified U937 cell line LDL binding assay.

doi:10.1016/s0009-8981(96)06409-1

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Article

Analysis of novel apolipoprotein B mutations using a modified U937 cell line LDL binding assay.

Clinica chimica acta; international journal of clinical chemistry - 9 Dec 1996

Meng Q H, Pajukanta P, Ilmonen M, Schuster H, Schewe C K, Andersson L C, Tikkanen M J

Abstract excerpt

The U937 myelomonocyte proliferation assay can be used to detect patients with familial defective apolipoprotein B-100 (FDB). Previous studies have employed electronic cell counting to assess cell proliferation. We simplified the assay using 3H-thymidine incorporation DNA analysis to measure cell...

Topics

Alleles
Apolipoproteins B
Biochemistry
Female
Humans
Lipoproteins, LDL
Lymphoma, Large B-Cell, Diffuse
Male
Mutation
Protein Binding
Tumor Cells, Cultured

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