Article
Cold-passaged human parainfluenza type 3 viruses contain ts and non-ts mutations leading to attenuation in rhesus monkeys.
Virus research - 1 Mar 1992
Hall S L, Stokes A, Tierney E L, London W T, Belshe R B, Newman F C, Murphy B R
Abstract excerpt
Cold-passaged (CP) mutants derived from the JS strain of wild type wt parainfluenza type 3 virus (PIV3) are being evaluated as candidate live virus vaccines. The wt virus was serially passaged 45 times at low temperature and mutant clones with the cold-adapted (CA), temperature-sensitive (ts), and attenuation (ATT) phenotypes were selected following passage levels 12, 18 and 45 (cp12, cp18, and cp45). The cp45...
Topics
- Animals
- Cell Line
- Evaluation Studies as Topic
- Humans
- Macaca mulatta
- Mutation
- Pan troglodytes
- Parainfluenza Virus 3, Human
- Paramyxoviridae Infections
- Phenotype
- Serial Passage