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Article

Construction of isogenic urease-negative mutants of Helicobacter pylori by allelic exchange.

Journal of bacteriology - 1 Jul 1992

Ferrero R L, Cussac V, Courcoux P, Labigne A

Abstract excerpt

Isogenic urease-negative mutants of Helicobacter pylori were constructed by allelic replacement. A region of cloned H. pylori DNA containing the structural urease genes (ureA and ureB) was disrupted by insertion of a mini-Tn3-Km transposon. Electrotransformation of H. pylori cells with kanamycin-ureB-disrupted derivative plasmids resulted in isolation of kanamycin-resistant H. pylori transformants. Competence for...

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Identifiers

PMID: 1320607
DOI: 10.1128/jb.174.13.4212-4217.1992
PMCID: PMC206198

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Source receipt: pubmed, retrieved 7 Sept 2026.

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