Construction of isogenic urease-negative mutants of Helicobacter pylori by allelic exchange.

doi:10.1128/jb.174.13.4212-4217.1992

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Article

Construction of isogenic urease-negative mutants of Helicobacter pylori by allelic exchange.

Journal of bacteriology - 1 Jul 1992

Ferrero R L, Cussac V, Courcoux P, Labigne A

Abstract excerpt

Isogenic urease-negative mutants of Helicobacter pylori were constructed by allelic replacement. A region of cloned H. pylori DNA containing the structural urease genes (ureA and ureB) was disrupted by insertion of a mini-Tn3-Km transposon. Electrotransformation of H. pylori cells with kanamycin-ureB-disrupted derivative plasmids resulted in isolation of kanamycin-resistant H. pylori transformants. Competence for...

Topics

Alleles
Blotting, Southern
Blotting, Western
Cloning, Molecular
DNA
DNA Transposable Elements
DNA, Bacterial
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Genes, Bacterial
Helicobacter pylori

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Weight

Mutagenesis, Insertional

Plasmids

Recombinant Proteins

Restriction Mapping

Urease

Source

Open in PubMed Open full text

Identifiers

PMID: 1320607
DOI: 10.1128/jb.174.13.4212-4217.1992
PMCID: PMC206198

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1320607-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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