A urease-negative mutant of Helicobacter pylori constructed by allelic exchange mutagenesis lacks the ability to colonize the nude mouse stomach.

doi:10.1128/iai.62.8.3586-3589.1994

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Article

A urease-negative mutant of Helicobacter pylori constructed by allelic exchange mutagenesis lacks the ability to colonize the nude mouse stomach.

Infection and immunity - 1 Aug 1994

Tsuda M, Karita M, Morshed M G, Okita K, Nakazawa T

Abstract excerpt

The urease of Helicobacter pylori has been proposed to be one of its pathogenic factors. A kanamycin resistance determinant was inserted in a cloned urease gene, and transformation-mediated allelic exchange mutagenesis was carried out to introduce the disrupted gene into the corresponding wild-ty...

Topics

Alleles
Animals
Helicobacter pylori
Mice
Mice, Nude
Mutation
Stomach
Urease

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