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Optimized BlaM‐transposon shuttle mutagenesis of <i>Helicobacter pylori</i> allows the identification of novel genetic loci involved in bacterial virulence

1 Apr 1996

Abstract excerpt

Helicobacter pylori is an important etiologic agent of gastroduodenal disease in humans. In this report, we describe a general genetic approach for the identification of genes encoding exported proteins in H. pylori. The novel TnMax9 mini-blaM transposon was used for insertion mutagenesis of a H. pylori gene library established in Escherichia coli. A total of 192 E. coli clones expressing active beta-lactamase...

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PMID: 8733234
DOI: 10.1111/j.1365-2958.1996.tb02623.x

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