Essential role of urease in vitro and in vivo Helicobacter pylori colonization study using a wild-type and isogenic urease mutant strain.

Abstract excerpt

To determine the role of urease in colonization of Helicobacter pylori, we constructed a Ure- mutant by allelic exchange mutagenesis and examined the characteristics of the Ure- mutant and its parental strain. We also evaluated whether both strains would be able to colonize the stomach of nude mice. Kmr transformants obtained showed the Ure- phenotype, and one transformant, HPT73, was established as a knock-out...

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