Article
Mutational analysis of an actin-binding site of cofilin and characterization of chimeric proteins between cofilin and destrin.
The Journal of biological chemistry - 15 Apr 1992
Moriyama K, Yonezawa N, Sakai H, Yahara I, Nishida E
Abstract excerpt
Cofilin and destrin are two related low molecular weight mammalian actin-binding proteins. Cofilin is an F-actin side-binding and pH-dependent actin-depolymerizing protein, and destrin is a pH-independent actin-depolymerizing protein. We have introduced a few point mutations within an actin-binding sequence of cofilin. Biochemical analyses of these mutant proteins have clearly shown that Lys112 and Lys114 of...
Topics
- Actin Depolymerizing Factors
- Actins
- Base Sequence
- Binding Sites
- Carrier Proteins
- Cloning, Molecular
- Cytoskeletal Proteins
- Destrin
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Hydrogen-Ion Concentration