Article
Nuclear binding of purified retinoblastoma gene product is determined by cell cycle-regulated phosphorylation.
Molecular and cellular biology - 1 Feb 1992
Templeton D J
Abstract excerpt
The retinoblastoma tumor suppressor gene product (pRb) is a nuclear protein subject to cell cycle-regulated hyperphosphorylation. I constructed a recombinant vaccinia virus vector that expresses both the underphosphorylated and hyperphosphorylated forms of pRb and purified the recombinant protein...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- CDC2 Protein Kinase
- Cell Cycle
- Cell Line
- Cell Nucleus
- Chromatography, Affinity
- Gene Expression Regulation
- Genes, Tumor Suppressor
- Humans
- Molecular Sequence Data
- Mutation
- Phosphoric Monoester Hydrolases
- Phosphorylation
- Recombinant Proteins
- Retinoblastoma Protein
- Vaccinia virus