Article
Cumulative effect of phosphorylation of pRB on regulation of E2F activity.
Molecular and cellular biology - 1 May 1999
Brown V D, Phillips R A, Gallie B L
Abstract excerpt
The product of the retinoblastoma susceptibility gene, pRB, is a nuclear phosphoprotein that controls cell growth by binding to and suppressing the activities of transcription factors such as the E2F family. Transactivation activity is inhibited when E2F is bound to hypophosphorylated pRB and released when pRB is phosphorylated by cyclin-dependent kinases (CDKs). To determine which of 16 potential CDK...
Topics
- Animals
- Binding Sites
- CDC2-CDC28 Kinases
- COS Cells
- Carrier Proteins
- Cell Cycle Proteins
- Cyclin E
- Cyclin-Dependent Kinase 2
- Cyclin-Dependent Kinases
- DNA-Binding Proteins
- E2F Transcription Factors
- Gene Expression Regulation
- Genes, Reporter
- Mice
- Mutagenesis, Site-Directed