Article
G1/S phosphorylation of the retinoblastoma protein is associated with an altered affinity for the nuclear compartment.
Cell - 3 May 1991
Mittnacht S, Weinberg R A
Abstract excerpt
Hyperphosphorylation of the retinoblastoma protein (pRB) is assumed to be a regulatory event leading to the inactivation of its growth-repressing functions. We demonstrate a functional alteration linked to the phosphorylation status of the protein. The un- or under-phosphorylated species are tightly associated with the nuclear structure. The association is resistant to digestion with nucleases, and release...
Topics
- Cell Line
- Cell Nucleus
- Deoxyribonuclease I
- Fluorescent Antibody Technique
- G1 Phase
- Humans
- Mutation
- Phosphorylation
- Retinoblastoma Protein
- S Phase
- Subcellular Fractions
- Tumor Cells, Cultured